Search academic texts

Information × Registration Number 0215U008573, 0115U004187 , R & D reports Title Using molecular and cellular technologies for biotech wheat and maize plants resistant to the herbicide glyphosate obtaining popup.stage_title Head Morgun Bogdan Volodymyrovych, Registration Date 29-12-2015 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 The explants for Agrobacterium-mediated transformation of cereals in vitro have been selected – calli of apical origin for the transformation of wheat and immature embryos to transform maize. The content of media for wheat regeneration has been optimazed – regeneration frequency reached 30,7±0,6% on modified MS medium supplemented with 0.5 mg/l BAP and 0.15 mg/l picloram. The antibiotics (timentin and ceftriaxone) have been chosen which both eliminate agrobacteria and enhance regeneration in wheat. As a result of Agrobacterium-mediated genetic transformation of immature maize embryos (3030 pcs.) of five genotypes with vector constructs containing glyphosate resistance gene CP4 epsps the regenerated plants were obtained on selective media. Workload has been performed to develop genetic constructs bearing glyphosate resistance genes. The approbation of vectors for plant genetic transformation with glyphosate resistance genes was done in tobacco. Product Description popup.authors Банникова Марія Олександрівна Горбатюк Ірина Романівна Нітовська Ірина Олександрівна Тараненко Артем Миколайович popup.nrat_date 2020-04-02 Close