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Information × Registration Number 0215U008626, 0115U001403 , R & D reports Title Establishment and characterization of cell based co-culture model of tumor and stromal cells for anticancer agents effectiveness evaluation popup.stage_title Head Filonenko Valeriy Viktorovitch, Доктор біологічних наук Registration Date 30-12-2015 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The work conditions were optimized and prepared primary cultures of human dermal fibroblasts. By imunohystyochemical and immunofluorescence analysis it was demonstrated that all of the cultured cells contained vimentin and fibroblast surface marker, but were not contaminated by cells of other types. In addition, primary culture of tumor associated fibroblasts was generated from samples of postoperative material of breast tumor. Also, the primary cultures of tumor cells have been received from the same material. For this method of enzymatic dissociation which consisted of the additional filtration of the suspension assemblies laktotsitov to get rid of large pieces of connective tissue and individual cells has been optimized. As a result primary cultures of breast cancer cells were obtained and their epithelial origin was confirmed by imunohystyochemical analysois of cytokeratins content. Product Description popup.authors Філоненко Валерій Вікторович Хоруженко Антоніна Іванівна popup.nrat_date 2020-04-02 Close