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Information × Registration Number 0215U008628, 0110U000692 , R & D reports Title Peculiarities of the functioning of mTOR-dependent signaling pathways: the multiplicity of mTOR isoforms and the regulation of metabolic processes in the cell popup.stage_title Head Filonenko Valeriy Viktorovitch, Registration Date 30-12-2015 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The existence of a hypothetical form mTOR 28-58 was confirmed by PCR analysis of cDNA HEK293 cells, MCF, Jurkat. Polyclonal antibodies against the unique C-terminal sequence 28-58 against mTOR N-terminal region common to mTOR wildtype form were obtained. For determination the functional activity of mTOR isoforms 28-58 based the stable HEK293 cell lines expressing mTOR isoform, which is recognized PolyAT against the N-terminal and C-terminal unique sequence were established. the colony formation assay was conducted for determining the change in proliferation under standard conditions. With the use of artificially synthesized methylated C-terminal peptides S6 kinase were founded that methylation of S6K promotes the interaction the protein kinase with TDRD7 Product Description popup.authors Гаріфулін Олег Мінірович Кіямова Рамзія Галямівна Маланчук Оксана Миколаївна Овчаренко Галина Вікторівна Пальчевський Сергій Станіславович Савінська Лілія Олексіївна Скороход Олександр Миколайович Тихонкова Ірина Олександрівна Хоруженко Антоніа Іванівна Чередник Ольга Володимирывна popup.nrat_date 2020-04-02 Close