Search academic texts

Information × Registration Number 0215U008629, 0110U000692 , R & D reports Title Peculiarities of the functioning of mTOR-dependent signaling pathways: the multiplicity of mTOR isoforms and the regulation of metabolic processes in the cell popup.stage_title Head Filonenko Valeriy Viktorovitch, Registration Date 30-12-2015 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Polyclonal antibodies against recombinant C-terminal peptides of new isoforms of mTOR (28-58, 27-58) were obtained. By using the obtained antibodies investigated the expression level of mTOR forms in the lysates of various cell lines. proteins with the expected molecular weight were identified. anti-oncogenic properties of mTOR / ?27-58 was shown in model cells. The subcellular localization of mTOR kinase phosphorylated at serine 2481 was investigated. The localization of mTOR (2481Ser) was determained in the middle of the metaphase plate as a discrete granules associated with chromatin Monoclonal antibodies against coenzyme A (CoA) were obtained and characterized. New high molecular weight form of the enzyme was found in nuclear. It is shown that it is less stable than known cytoplasmic one and, moreover, its expression level is regulated by growth factors. Product Description popup.authors Гаріфулін Олег Мінірович Кіямова Рамзія Галямівна Маланчук Оксана Миколаївна Овчаренко Галина Вікторівна Пальчевський Сергій Станіславович Савінська Лілія Олексіївна Скороход Олександр Миколайович Тихонкова Ірина Олександрівна Хоруженко Антоніа Іванівна Чередник Ольга Володимирывна popup.nrat_date 2020-04-02 Close