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Registration Number

0216U000059, 0115U007215 , R & D reports

Title

Novel electrichemical nanosensors for analysis of toxic ions.

Head

Gonchar Mykhailo Vasylyovych, Кандидат педагогічних наук

Registration Date

19-01-2016

Organization

Institute of cell biology, National Academy of Sciences of Ukraine

Description

Research objectives: iron and manganese ions, characterization of nanomaterials, biosensors, enzymatic analysis. Aim: To establish of modern electrochemical nanosensors for detection of toxic ions. The aim of the stage for 2015: A synthesis and characterisation of nanomaterials and construction of amperometric microelectrodes for analysis of iron and manganese ions. Methods: microbiological, biochemical, physical, chemical, enzymatic, electrochemical, biosensor. Mono- and hybrid nanoparticles of gold and silver were obtained by reduction of HAuCl(4) and AgNO(3). A study of the structure and morphology of synthesized nanomaterials were performed using scanning electron microscopy, atomic force microscopy and fluorescence microscopy. It was shown, that dezferal as a natural iron-selective ionophore can be used as a selective element of amperometric biosensor for determination of iron ions. It was shown, that a covering surface of gold planar microelectrodes by gold nanoparticles significantly increases the sensitivity of the sensor. For nano-modified gold electrode amperometric output increased for 43 folds - to Fe(III)-ions and 136 folds - for Fe(II)-ions. A fundamentally new method of enzymatic determination of manganese(II) ions using apoenzyme of recombinant human arginase I for fluorymetric (EF) and spectrophotometric (ES) analysis was proposed. The detection of reaction product of arginine-hydrolysis - urea at the presence of reconstructed Mn(II)-consisted holoenzyme was performed. This is selective and simple method. A linear range for EF is 0.5 - 16 pM, and the limit of detection - 0.2 pM Mn(II) in the reaction mixture. New method was successfully tested on the samples of wastewater of mines for mining of nonferrous and noble metals. It was shown the high level of correlation between results of the new method and atomic absorption spectroscopy (R = 0,999). According to the results of the research in 2015 were printed five articles in peer-reviewed international journals and 2 abstracts, two scientific articles were submitted for publication.

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Гайда Г.З.
Гончар М.В.
Закальська О.М.
Закальський А.Є.
Лаврик М.І.
Прокопів Т.М.
Синенька М.М.
Смуток О.В.
Стасюк Н.Є.