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Information × Registration Number 0216U000706, 0111U006097 , R & D reports Title Screening of crops breeding material for genetically modified construction presence and development of recipient systems popup.stage_title Head Volkova Natalіya Eduardivna, Доктор біологічних наук Registration Date 19-02-2016 Organization Plant breeding and genetics institute-National center of seed and cultivar investigation popup.description2 Object of research - morphogenesis in vitro of soybean plants tissue, screening samples for the presence of transgenic constructs. The aim - to test varieties, lines, hybrids of wheat, barley, maize, soybean of PBGI for presence of transgenic sequences. Selection of the optimal nutrient composition for morphogenic callus culture and regeneration of soybean genotypes. Methods - polymerase chain reaction, gel electrophoresis, methods tissue culture in vitro. 10 wheat, 12 soybean, nine barley varieties, nine maize lines and 20 hybrids of PBGI breeding were tested: they were free of the most common modern commercial lines of GM transgenic regulatory elements (CaMV 35S promoter , PSsuAra, Ta29, nos terminator). Morphogenesis features in vitro tissue 12 soybean varieties southern Ukraine were teasted. Most suitable explants - cotyledon nodes - for morphogenic callus were chosen. The technology of obtaining of soybean regenerants Syayvo variety from callus. Can be used as the optimum system for transgenesis recipient Product Description popup.authors Волкова Наталія Едуардівна popup.nrat_date 2020-04-02 Close