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Registration Number

0216U000711, 0111U006104 , R & D reports

Title

DNA technology for crops varieties, lines, hybrids identification, development of molecular genetic passports and the completion of DNA typing database.

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Head

Volkova Nataliya Eduardivna, Доктор біологічних наук

Registration Date

19-02-2016

Organization

Plant breeding and genetics institute-National center of seed and cultivar investigation

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Object - molecular-genetic polymorphism of wheat, barley, maize, sunflower. Purpose - DNA typing of varieties, lines, hybrids of wheat, barley, maize, sunflower. Methods - polymerase chain reaction, fragment-analysis, gel electrophoresis. 69 samples of wheat varieties were differentiated and identified by the results of microsatellite (MS) analysis of 1-9 loci. Formula wheat genotypes were developed by MS analysis. Number and structure of molecular markers were optimized for cultivar control of breeding and seed nurseries. Cluster analysis of lines allocated 49 soft wheat, according to the allelic composition of the 17 MS loci and morphological characters was carried out. According to PCR loci Xgwm18, Taglgap, Scm9 varieties and lines Pavon ma, Salmon, H242 / 97-2, H273 / 97, CWXs are 1RS.1BL translocation, in which the genetic polymorphism was identified; this indicates that the identified translocations are different. DNA typing of 33 barley varieties by MS loci was done. Genetic passports of winter barley varieties from different institutions were issued. The database is updated with the DNA-typing information about the characteristics of MS allelic loci of barley varieties. DNA typing of sunflower F1 hybrids and inbred lines was conducted by MS loci identification test panel. According to the results of DNA typing of 14 MS loci molecular genetic formula of sunflower hybrids and lines were fixed. To increase the test panel identifying capacity 23 MS locus were investigated from which selected seven. Allelic composition of 13 MS loci for 25 maize hybrids were established. Molecular genetic formulas were recorded and molecular genetic passports were issued for 17 maize hybrids

Product Description

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Волкова Наталія Едуардівна
Солоденко Анжелла Євгенівна

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2020-04-02