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Information × Registration Number 0216U001114, 0111U006017 , R & D reports Title Peculiarities of haploid plant development in rapeseed on the basis of experimental haploidy popup.stage_title Head Soroka Anatoliy Ivanovich, Доктор сільськогосподарських наук Registration Date 22-01-2016 Organization I popup.description2 The object of study: the process of induction of morphogenic structures in anther culture of breeding accessions in rape. The purpose - to study the influence of various factors on the formation of morphogenic structures of various origin and development of optimized methods for obtaining the double haploids in rapeseed through anther culture, as well as methods for identifying regenerated plants of different ploidy level. The methods of research - experimental laboratory research, analytical and statistical and mathematical analysis. Cytokinin BA, without combination with other phytohormones, possesses the best action for the induction of morphogenic structures of gametophytic origin. The addition of 2,4-D into the medium together with BA or presence of 2,4-D alone leads to the formation of morphogenic structures from diploid anther tissues. This technique allows to obtain double haploids of rapeseed with a high rate, and the lack of negative impact of the D-5A thickening agent allows to use it as a substitute for agar in the preparation of culture media for the cultivation of rapeseed anthers in vitro. Area of implementation - biotechnological divisions in breeding institutions of various forms of ownership. Product Description popup.authors Бойко Наталія Вікторівна Сорока Анатолій Іванович popup.nrat_date 2020-04-02 Close