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Information × Registration Number 0216U001132, 0113U002152 , R & D reports Title To develop medico-physical bases of magnetic nanotherapy technology for malignant neoplasms popup.stage_title Head Orel Valery Emmanuilovich, Доктор біологічних наук Registration Date 25-01-2016 Organization Ukrainian Research Institute of Oncology and Radiology popup.description2 The object of study - magnetic nanocomplex of Fe3O4 or Fe2O3 nanoparticles and doxorubicin; localizers cell lines: T47D - human breast carcinoma and HeLa S3 - cervical cancer, A-549 - human lung cancer and rat fibroblasts, animals with transplanted tumors - carcinosarcoma Walker 256 Guerin carcinoma. Equipment - mechano-magneto-chemical reactor; apparatus for local shortwave inductothermy "Magnitherm" (Radmir, Ukraine); mercury thermometer S195 (Medisana AG, Hilden, Germany), 300 mm metal line measuring. Purpose - to develop medical and physical bases of magnetic nanotherapy based on the effect of the joint action of the inhomogeneous constant magnetic field and radiofrequency hyperthermia and magnetic nanocomplexes in animal models of cancer. Research methods - physical, technical, physical and chemical, immunocytochemical, laboratory and statistical. The design of mechano-magneto-chemical reactor, which includes linear motor and the generator of electromagnetic field with a sinusoidal waveform, was upgraded. Magnetic dipole applicator for apparatus "Magniterm" and stand for simulating the mechanical vibrations of nanoparticles were developed and manufactured. It was shown that the area size and spatial distribution of the nanoparticles was dependent on the magnetic force lines of applicator. The technology of magnetic nanotherapy was experimentally proved on tumor cell cultures. When magnetic-dipole applicator for electromagnetic irradiation with pin localizers and magnetic nanocomplex composed of Fe3O4 nanoparticles and doxorubicin were used in the combined treatment of animals with carcinosarcoma Walker 256 carcinoma Guerin during moderate radiofrequency hyperthermia, the tumor growth inhibition rate was correspondingly higher by 2.9 times and 28% as compared to the treatment by officinal doxorubicin and electromagnetic irradiation. The mechanisms of antitumor effect of the technology of magnetic nanotherapy were investigated on the model of cell cultures of human cancer T47D, HeLa S3 and A-549. Immune marker analysis of protein p21WAF1, beta-catenin and topoisomerase II alpha showed that nanotherapy during moderate radiofrequency hyperthermia causes relocalization of specific antigens depending on the cell type. A computer model of the synthesis of magnetosensitive nanocomplex in nano-reactor based on the initiation of free radicals in the process of mechano-magneto-chemical synthesis and effects of inhomogeneous constant magnetic and electromagnetic irradiation on the antitumor activity of magnetic nanocomplex in the conditions of oxidative stress during moderate radiofrequency hyperthermia was developed. Field of application - oncology. EXPERIMENTAL MODELS OF CANCER, MAGNETIC NANOCOMPLEXES, CONSTANT MAGNETIC FIELD, RADIOFREQUENCY HYPERTHERMIA. Product Description popup.authors Бездєнєжних Н.О. Дзятковська І.І. Крючков Є.І. Рихальський О.Ю. Романов А.В. popup.nrat_date 2020-04-02 Close