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Information × Registration Number 0216U001133, 0113U002146 , R & D reports Title To develop technology of reception melanoma-specific transferfactor polypeptides and to prove expediency of their application for skin melanoma patients popup.stage_title Head Filchakov Feodosij V., Registration Date 25-01-2016 Organization National Cancer Institute popup.description2 Object of research: immune cells, models of experimental tumors, low molecular weight extracts of lymphocytes. Purpose -To prove experimentally the new approach to treatment of skin melanoma patients by carrying over tumor-specific immunoreactivity by transferfactor polypeptides (TFP). Methods of investigation - immunological, flow cytometry, the method of cell culture, experimental, statistical. Equipment: centrifuge OPN-3, OPN-8; microscope; flowing cytometer FACScan; the automatic sampleres; thermostats; CO2 incubator, pH meter. In accordance with technology developed samples obtained low molecular weight melanoma-nonspecific and specific TFP in different experimental systems investigated their immunotropic properties and antitumor activity in an experimental model of B16 melanoma in C57BL / 6 mice.It is found that the melanoma-specific TFP, regardless of origin, capable of transferring in vitro and in vivo immunoreactivity of B16 melanoma antigens sensitized lymphocytes. For a single prophylactic administration of melanoma-specific samples of TFP (rat and fetal) and non-specific fetal sample TFP show a protective anti-metastatic effect. Regular introduction of specific TFP (regardless of origin) or non-specific TFP embryonic origin significantly increases the life expectancy C57BL / 6 mice model of passive B16 melanoma metastasis by reducing the frequency of metastasis, reduction of the number and size of pulmonary metastases. The dialysate human leukocyte (preparation TFP), prevents the development of deep disturbances in the immune system of patients with generalized melanoma during chemoimmunotherapy, resulting in restoration of the number of peripheral blood lymphocytes (including subpopulations of cytotoxic T-lymphocytes), reduction in circulation amount of regulatory T-lymphocytes and preserving the functional activity of the T-cell population. GENERALIZED SKIN MELANOMA, TRANSFER FACTOR POLYPEPTIDES , CHEMOIMMUNOTHERAPY, an EXPERIMENTAL MODEL of B16 MELANOMA, ANTIMETASTATIC EFFECTS Product Description popup.authors Кукушкіна С.М. Льон Г.Д. Шуміліна К.С. popup.nrat_date 2020-04-02 Close