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Information × Registration Number 0216U001336, 0111U002744 , R & D reports Title On the basis of molecular markers and androgenesis to develop technology of winter triticale marker selection. popup.stage_title Head Serhiy Voloshchuk, Registration Date 05-02-2016 Organization The V.M. Remeslo Myronivka Institute of Wheat of National Academy of Agrarian Sciences of Ukraine popup.description2 Object of study: varieties and hybrids of winter triticale, winter triticale androgenic cultureы, DNA markers for wheat and rye chromosomes. The purpose. To develop method of production of dihaploid lines, to choose molecular markers suitable to creation of isogenic on D / R substitution lines and markers to analyze genetic diversity of winter triticale. Methods: tissue culture, PCR analysis, statistical analysis, field experiments, laboratory methods. Results. Methods for culturing winter triticale isolated microspores were optimized and methods of dihaploid lines in vitro producing were improved. Series of D / R substituted lines were developed. Molecular SSR markers of rye R genome suitable for studying the genetic variability of winter triticale varieties were determined. Screening collections of winter triticale and hybrid populations and dihaploid lines for 2R / 2D-substitution and allelic state of Rht1 and Vp1 genes as well as SSR-markers of QTL and indices of resistance to sprouting was conducted. In hybrid combinations strong influence of 2R / 2D-chromosome substitution on winter triticale plant height, seed dormancy, resistance to sprouting, elements of grain productivity was revealed. Field of use; genetics, plant breeding. Product Description popup.authors Волощук Ганна Дмитрівна Волощук Сергій Іванович Пикало Сергій Володимирович Харченко Михайло Володимирович popup.nrat_date 2020-04-02 Close