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Information × Registration Number 0216U001610, 0115U005020 , R & D reports Title Search candidate genes оf resistance to stem rust in cultivated and wild grasses for their further use in wheat breeding popup.stage_title Head Blum Yaroslav Borysovich, Доктор біологічних наук Registration Date 09-03-2016 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 The presence of barley Rpg5, RGA1 and Adf3 resistance gene homologues, which provide resistance to stem rust in cereal species such as Triticum aestivum, T. urartu, Aegilops tauschii, Oryza sativa, Sorghum bicolor, Thinopyrum elongatum, Brachypodium distachyon, Eletrigia repens and Setaria italica, was analysed. In case of Adf3 gene, the close homologues were found in Th. elongatum and T. aestivum. Only two sequences which have the same length as Rpg5 gene protein were found in T. urartu and T. aestivum, but their kinase domains differ significantly from that of Rpg5 gene protein. The highly-identical sequences for complete RGA1 gene protein product in T. urartu and T. aestivum were identified. Based on sequences of found homologous, the primers were synthesized. Using these primers, the fragments of Rpg5 gene homologous were identified in the genomes of T. aestivum and E. repens. The identified sequences were sequenced, and their further analysis is going to help in evaluation of Rpg5, RGA1, Adf3 gene homolog interaction efficiency in the resistance processes to stem rust, particularly Ug99 strain, in wheat. Product Description popup.authors Іващук Богдан Володимирович Блюм Ярослав Борисович Калафат Любов Олександрівна Пірко Ярослав Васильович СпівакСвітлана Ігорівна popup.nrat_date 2020-04-02 Close