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Information × Registration Number 0216U001612, 0115U005021 , R & D reports Title Lactoferrin gene application for creation of pathogen-resistant plant lines of Solanaceae family popup.stage_title Head Yemets Alla Ivanivna, Доктор біологічних наук Registration Date 09-03-2016 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 The efficacy of supervirulent A. tumefaciens strain EHA105 carrying the plasmid vector pBIN35LF which contains human lactoferrin gene hLf driven under the control of cauliflower mosaic virus 35S promoter and nopaline synthase terminator, and selectable marker neomycin phosphotransferase II (nptII) gene conferring resistance to kanamycin was tested for genetic transformation of S. tuberosum и L. esculentum. In vitro tissue cultures of Ukrainian varieties of tomato and potato have been were established; their plantlet regeneration and rooting potential in vitro has been studied. The method of Agrobacterium--mediated transformation was adapted for L. esculentum, the selection of transgenic lines expressing human lactoferrin gene was conducted out, morphological traits of these lines were studied. Product Description popup.authors Блюм Ярослав Борисович Бузіашвілі А.Ю. Мельничук О.В. popup.nrat_date 2020-04-02 Close