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Information × Registration Number 0216U001752, 0113U001517 , R & D reports Title Biological bases for synbiotic comolexes development using electromagnetic and ultrasonic waves popup.stage_title Head Babych Evgenij Mihailovich, Доктор технічних наук Registration Date 04-04-2016 Organization The Mechnikov Research Institute of microbiology and immunology at Kharciv popup.description2 Object of study: probiotic properties of Lactobacillus spp. strains, Lactobacilli metabolites, bacterial biofilms biological properties of microorganisms (catabolic, adhesive, lecitinase, plasmocoagulase). Objective: To study fundamental hypothesis about the ability of electromagnetic and ultrasonic waves to influence the formation of microbial biologically active substances and biofilms. Methods: microbiological, biological, statistical. The researches allowed to identify the strain L. plantarum isolated from bees, which manifests antagonistic activity against pathogenic corynebacteria 1,8-5,0 times more often than the basis probiotic strains L. acidophilus and L. ramnosus. Physicochemical factors that enhance or inhibit antagonistic and adhesive properties of lactobacilli were defined. It was found that the microaerofilic conditions increased the rate of glucose consumed by lactobacilli and stimulated their competetive and adhesive properties. It was established that the use of millimeter waves in 61,0 GHz frequency band increased competitive properties of candidate-strain against pathogenic corynebacteria and Staphylococcus aureus, and low-energy ultrasound did not influence on the competitive properties. The influence of probiotic strain-candidate metabolites on biological properties of pathogenic (C. diphtheriae) and opportunistic (S. aureus) bacteria was studied. It was found that these substances inhibited the growth of microorganisms and the formation of bacterial biofilms, helped to reduce the toxicity of diphtheria exotoxin and repressed formation of the enzymes of Staphylococcus aureus aggression . The results reveal the prospects of development of technologies aimed at creating of a new type of drugs against C. diphtheriae and S. aureus, and improve their production and testing. Product Description popup.authors Антушева Тетяна Іванівна Бабич Євгеній Михайлович Ждамарова Лариса Анатолівна Калініченко Світлана Вікторівна Рижкова Тетяна Анатолівна Скляр Надія Іванівна Шкодовська Наталья Юріївна popup.nrat_date 2020-04-02 Close