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Information × Registration Number 0216U002027, 0115U001971 , R & D reports Title Investigations of L-and D-lactate: cytochrome c oxidoreductases isolated from the recombinant yeast strains of Hansenula polymorpha and their use for the construction of amperometric biosensors. popup.stage_title Head Gonchar Mykhailo Vasylyovych, Кандидат педагогічних наук Registration Date 18-01-2016 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Research Objects : L- lactate, D-lactate, characteristics, bioselective elements amperometric biosensors. The aim: to investigate the physico-chemical and kinetic characteristics of L- and D-lactate: cytochrome c oxidoreductases, which were isolated from recombinant yeast strains Hansenula polymorpha. To develop of biosensors on based on to the enzymes. The aim of stage of 2015: to optimize a synthesis, separation, purification and characterisation of L- and D-lactate selective flavocytochromes obtained from recombinant Hansenula polymorpha yeast strains and their usage as biosensor's bioelements. Methods: microbiological, biochemical, physical, chemical, enzymatic, electrochemical, biosensoric. The optimisation of synthesis of enzymes: L-lactate: cytochrome c oxidoreductase (flavotsytohromu b2, FC b2), and D-lactate: cytochrome c oxidoreductase (DLDH) in recombinant yeast strains Hansenula polymorpha - overproducer of appropriate enzymes has been performed. The schemes of purification of FC b2 and DLDH from H. polymorpha have been developed and preparative quantities of highly purified enzymes have been obtained. The laboratory prototype of electrochemical biosensors for determination of lactate in the samples of biological liquids, based on the purified preparations of FC b2 and nanoparticles of noble metals has been constructed. Product Description popup.authors М.І. Лаврик М.В. Гончар О.В. Смуток popup.nrat_date 2020-04-02 Close