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Registration Number

0216U002032, 0111U003187 , R & D reports

Title

Studying of theoretical bases for drought resistance and developing of estimation methods of sugar beet plants, which are tolerant to the drought using biotechnology methods

Head

Petyuh Grygoriy Pavlovich,

Registration Date

18-01-2016

Organization

Institute of a sugar-beet

Description

Object of research - the processes of change physiological state of sugar beet plants which are characterized by different levels of tolerance to drought, under the influence of stress factors such as water deficit conditions. Purpose - to study the biological characteristics of sugar beet plants at different levels of their organization as well as their reactions to the factors of drought and also a development of methodological basis of evaluation of plants tolerance to drought in vitro conditions. Research methods - laboratory methods: methods of plant propagation in vitro conditions, methods of molecular and biochemical as well as cytological analysis; statistical methods (cluster analysis, calculation of correlation and regression, disperse analysis). Main results and their novelty - a theoretical summarizing to develop ways of determining sugar beet plants to drought tolerance in order to further their selection, formulated a fundamentally new approach to solve this problem and performed its scientific justification, demonstrated the mechanisms of intracellular protecting sugar beet plants to stress under water deficit conditions as one of the main factors of drought. For the first time in Ukraine and CIS it was developed and experimentally verified method for rapid assessment of sugar beet plants using the methods of in vitro culture, which allows to evaluate the plant material sugar beet any origin tolerance to water shortages (drought) during several months, which will reduce the breeding process on the basis of a few years. We propose a model way to evaluate genotypes of sugar beet plants to determine their levels of tolerance to the water deficit conditions using in vitro culture, which includes the use of special culture media for the cultivation and propagation of plant clones, and also allows to differentiate genotypes on the basis of increased adaptive capacity to stress factors by calibration feature of their cultivation in these environments. Patterns found in the dynamics of intracellular components of the antioxidant protection of sugar beet plants at their cultivation in the osmotic stress conditions. Keywords: sugar beet, tolerance to water deficit, drought, morphophysiologic analysis, biotechnological methods of assessment, the antioxidant defense system.

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Дворак Катерина Петрівна
Петюх Григорій Павлович
Черидничок Оксана Іванівна