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Information × Registration Number 0216U002245, 0113U001231 , R & D reports Title Optimization of heterologous expression conditions in plants for the production of commercially valuable substances popup.stage_title Head Kuchuk Mykola Viktorovych, Registration Date 08-02-2016 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 A strategy for genetic vectors design to optimize expression of transgenes has been developed. Vectors for genetic transformation of plants that provide accumulation reporter protein GFP in different compartments of the plant cell (apoplast, endoplasmic reticulum, vacuoles and chloroplasts) as a vectors with reporter gene under control of inducible promoters were created. transgenic plants of broccoli (Brassica oleracea L. var. Italica), alfalfa (Medicago sativa L.), peanuts (Arachis hypogaea L.), pea (Pisum.sativum L.) and trasplastomic lines of tobacco (Nicotiana tabacum L.) containing leukocyte interferon gene inf-?2b; transgenic plants of strawberry (Fragaria x ananassa) and lettuce (Lactuca sativa L.), containing gene of sweet protein thaumatin II connected with signal of chloroplast targeting; transgenic plants of alfalfa, peanut and clover (Trifolium pretense L.), containing the gene of the herbicide Pursuit resistance; transgenic potato plants (Solanum tuberosum L.), containing in its genome a pal gene; transgenic plants of alfalfa and peanuts, which contain connected genes of ESAT6 and Ag85b antigens, stimulating the immune defense against tuberculosis were obtained. The influence of various sugars presence in the culture medium on the qualitative and quantitative composition of Selosia cristata callus nitrogenous pigments betalains and the impact of elicitors and the presence of illumination on content of meroterpen bakuchiol in Psoralea ensifolia and Psoralea drupaceae shoots was found. Product Description popup.authors Белокурова Валерія Борисівна Василенко Максим Юрійович Кіщенко Олена Михайлівна Листван Катерина Володимирівна Мазур Марія Григорівна Ніфантова Світлана Миколаївна Петерсон Антон Андрійович Рудас Володимир Андрійович Шелудько Юрій Всеволодович Щербак Наталія Леонідівна popup.nrat_date 2020-04-02 Close