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Information × Registration Number 0216U003066, 0115U002952 , R & D reports Title Development, testing and production of an enzymatic test kit "Arhitest" for the analysis of arginine in clinical samples. Section 2. Testing of a kit on serum samples of human and laboratory animals popup.stage_title Head Korpan Yaroslav, Кандидат біологічних наук Registration Date 12-01-2016 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 In collaboration with the Institute of Cell Biology of the National Academy of Sciences of Ukraine the test series of enzymatic kit "Argitest" for analysis of arginine based on recombinant arginase have been developed. It has been established that arginase I of human liver is an optimal enzyme for the developmenyt of a diagnostic test kit with fluorometric arginine detection. Kit was tested on clinical samples of blood serum of different groups of donors - healthy donors, patients with liver disease, and athletes who use arginine-containing pills. It was shown that in the group of healthy patients L-Arg concentrations constituted from 0.087 to 0,159 mM, in the group of patients suffering from liver diseases - concentration of L-Arg was in the range of 0,167 to 0,284 mM, in the group of athletes taking arginine-containing pills L-arginine concentration was the highest and was in the range of 0.385 to 0.440 mM. These data correlate well with the literature, and the proposed method can be recommended for the development of appropriate government standards determining the content of L-Arg and production of a commercial test kit. Product Description popup.authors Єльська Г.В. Білоіван О.А. Жибак М.Т. Корпан Я.І. Солдаткін О.П. Чан Д.Х. popup.nrat_date 2020-04-02 Close