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Information × Registration Number 0216U003070, 0110U000694 , R & D reports Title Molecular chaperon as a universal regulators of stress-indused signalling pathways cardiomyocytes popup.stage_title Head Sidorik L.L., Registration Date 12-01-2016 Organization Institute of Molecular Biology and Genetics popup.description2 The simulation of molecular dynamics (MD) complex Hsp60-Akt1 in solution, under conditions that are close to physiological, a time interval of 20 ns, using the program NAMD. It is shown that due to the formation of complex Hsp60-Akt1, changing komformatsiya protein Akt1 with closed shape (activated) to open (activated) allowing protein kinases phosphorylate key activity for Akt1 amino acids, such as Thr 308. revealed that a stable connection is inactive The key rationale His194- Thr 308-Arg274-Asp292 change that provides access to phosphorylation of Thr 308. There were transfected with recombinant plasmid pcDNA3.1 / hsp60 mammalian cells, namely: HEK293, A431, HeLa, KTC-2, CHO, WRO to study the effects on mammalian cells superekspresiyi molecular chaperone Hsp60 expression and kinase Akt1 p70S6K1. It was chosen terms transfected cells and selected cell lines (HEK293, A431, WRO), which were the most resistant to transfect recombinant plasmid for selected conditions. Product Description popup.authors І.В. Крупська Бобик В.І. Вігонтіна О.Г. Капустян Л.М. Маркелова О.Ю. Рожко О.Т. Яковенко Л.Ф. popup.nrat_date 2020-04-02 Close