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Information × Registration Number 0216U003376, 0113U003065 , R & D reports Title Microorganisms intercellular communication functioning patterns study in presence of the synthetic and natural biologically active compounds popup.stage_title Head Filipova Tetiana Olegivna, Registration Date 18-02-2016 Organization Odessa I.I. Mechnikov National University popup.description2 A fundamentally new synthetic quinolone signal P. aeruginosa: 2-heptyl-3-hydroxy-4-quinolone (PQS). For the first time received structural analogs of different lengths acylic chain 2-octyl-3-hydroxy-4-quinolone, 2-lauryl-3-hydroxy-4-quinolone and 2-nonyl-3-hydroxy-4-quinolone. For the first time the influence of exogenous autoinduktoriv intercellular communication system P. aeruginosa synthesis ramnolipidiv and proved the possibility of their use in biotechnological production of biosurfaktantiv. Especially significant effect quinolone signal reveals that the concentration-dependent manner increases the synthesis ramnolipidiv 1.9; 3.3 and 5.2-fold in the presence of 40, 60 and 80 mM PQS. New important scientific result is the establishment of growth diramnolipidiv synthesis and, above all, Rha-Rha-C10-C10. This ratio diramnolipid / monoramnolipid (Rha-Rha-C10-C10 / Rha-C10-C10) increased from 2.1: 1 to control up to 3: 1, 3.6: 1 and 4.5: 1 at concentrations of 40 PQS 60 and 80 microns, respectively. Considering literature data on higher antitumor activity is diramnolipidiv obtained sample mixtures biosurfaktantiv P. aeruginosa is promising for the study of their pharmacological activity. The theoretical significance of the results is the assumption that the alarm is activated quinolone ramnozyltransferazy 2 by amplifying gene expression rhlC, which encodes the synthesis of the enzyme. In addition, there is a possibility of direct influence on the activity of PQS ramnozyltransferazy 2. For the first time found that one type autoinduktory psevdomonad particular PQS P. aeruginosa, may affect the functioning of other types of quorum sensing, biofilm shown to increase weight and pigment synthesis fenazynovyh strains of Pseudomonas aurefaciens and Pseudomonas fluorescens. For the first time findings about the impact of exogenous autoinduktora nizyna growth and biofilm formation of lactic acid bacteria: Lactococcus lactis and Lactobacillus plantarum. It was established that the autoinduktor, which is synthesized laktokokamy, has the same effect on both types - stimulates the formation of biofilms and exopolysaccharides synthesis at concentrations of 100-500 ng / ml. At the same time the presence of 5.10 mg / ml nizyna these processes are inhibited in 6-10 times. It is proved that the presence of exogenous autoinduktora laktokokiv in concentrations of 100-500 ng / ml of activated biosynthesis of antimicrobial compounds lactic acid bacteria. For the first time shown that under joint cultivation of lactic acid bacteria or the presence nizyna significantly inhibited the ability of Candida albicans to form biofilm psevdohify and form. These data show a decrease pathogenicity of yeasts under the influence of secondary metabolites lactic acid bacteria. Product Description popup.authors Галкін Борис Миколайович Галкін Микола Борисович Малярчик Ігор Олегович Русакова Марія Юріївна Семенець Анастасія Сергіївна popup.nrat_date 2020-04-02 Close