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Information × Registration Number 0216U003446, 0114U003172 , R & D reports Title To identify peculiarities of storage of plant gene pool accesses of field crops; to ensure maintenance of doublet seed repository of gene pool popup.stage_title Head Kharchenko Yurii Viktorovych, Registration Date 03-03-2016 Organization Ustymivka Experimental Station of Plant Production of Yurjev Plant Production Institute of NAAS popup.description2 Research Object - collections of cereal, legume, groat, technical and fodder crop, vegetable, maize and potato accessions, decorative plants (trees and shrubs of the State Arboretum "Ustymivskyy") of Ustymivka Experiment Plant Production Station. The aim was to reveal features and patterns of medium-term storage of agricultural crop seeds of collection accessions, ensuring medium-term ex-situ conservation of agricultural crop collections in the duplicate Depository of Ustymivka EPPS, delivery of certified seeds to the National Depository for long term storage. The aim involved solving a number of tasks: maintenance of viability and authenticity of seed collections of the Experiment Station; regeneration of seeds for the purpose of storage in the duplicate Depository of Ustymivka EPPS and the National Depository of Ukraine; determination of storage peculiarities of seed samples of different crops, depending on storage conditions and characteristics of genotypes; continued maintenance of electronic databases (introduction, passport, storage) to work with the plant gene pool; provision of breeding and research institutions of Ukraine with information on the plant gene pool. The total volume of the collection of Ustymivka EPPS is 28,750 accessions of 133 crops and 472 botanical species of the State Arboretum "Ustimivskyy." 28,461 accessions of the collection of Ustymivka EPPS, including 27,826 seed samples (of them, 15,887 accessions in the refrigerator), 635 potato accessions and 110 vegetable mother accessions are stored in the viable state. The germinability was monitored for 420 accessions, and their satisfactory viability was acknowledged. 7,758 accessions were grown to renew germinability and keep accessions alive. Seeds of 1,847 collection accessions were transferred in the National Depository for storage. Field and laboratory studies on the germination energy and and germinability (899 samples) of seeds of different crops, varieties, subspecies, environmental groups within these crops were conducted to explore characteristics of seed storage, depending on storage conditions and genotype peculiarities. Certain patterns to keep high viability of accession seeds of the most of collection crops of the Experimental Station for a long time (over 10 years) were revealed. Preparation and storage regimens of the potato collection were studied, and guidelines on these regimens were given. A multiyear experiment was laid out to determine an optimal level of moisture content in seeds for preservation of species diversity of vegetables and forage crops and storage of wheat working collections in the refrigerator. The passport database containing 29,216 accessions was submitted to the NCPGRU. Users got 3,280 collection accessions, including 14 special and 40 training collections (1,160 accessions of 65 crops). 22 catalogues of seeds, sheaves and fruit of 135 crops were compiled. 9 scientific papers were published. "Guidelines for Preparation and S were conducted Short-Term Storage of Seed Accessions of Working and Special Collections of Field Crops" were issued. The scientific-practical conference "Plant Genetic Resources for Breeding and Seed Production" was held. The products obtained from the project will ensure preservation of the plant gene pool of the National Collection, provide users with information on the genetic diversity of the collection material, provide breeding and research programs with top-quality seeds and plants, and training programs - with visual aids for teaching biological disciplines. The scientific products have no analogues in Ukraine Product Description popup.authors Іллічов Юрій Геннадійович Білик Олена Миколаївна Бондус Росина Олексіївна Вискуб Роман Станіславович Головаш Людмила Михайлівна Кір'ян Віктор Михайлович Кочерга Валентина Яківна Павлик Сергій Анатолійович Роговий Олександ Юрійович Силенко Олена Сергіївна Силенко Сергій Іванович Тригуб Олег Володимирович Харченко Любов Яківна Холод Сергій Григорович popup.nrat_date 2020-04-02 Close