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Information × Registration Number 0216U003480, 0113U000100 , R & D reports Title Study neuroprotective properties of stem cells in various experimental models of pathologies of the nervous system popup.stage_title Head Butenko Genadij Mikhailovich, Registration Date 28-01-2016 Organization Institute of genetic and regenerative medicine Acad.Med.Sci. of Ukraine popup.description2 Report about research work: figures, tables, 1 supplement, references Objects: FVB/N, 129/Sv and С57/Bl mice and Wistar rats The aim of the research was to study the neuroprotective effect of the transplantation of various stem cells and searching for the new methods and approaches for its increase using experimental models (in vivo, in vitro) of the nervous system pathology. Models: neurotoxic models of the demyelinization and hemiparkinsonism, sciatic nerve resection, stem cell transplantation, human Leukemia Inhibitory Factor (LIF) and melatonin administration, hippocampal oxigen-glucose deprivation in vitro (OGP). Methods: cultural, histological, confocal and electronic microscopy, biochemical, open field test and flow cytometry. Equipments: CO2-incubator, laminar-flow box, FACSAria, laboratory centrifuge, microscope, pH instrument and balance. Results. Transplanted into hippocamp neural stem cells (NSCs) after OGP differentiate into mature neurons generating synapses with neuronal cells of the recipient tissues. LIF injection to the cuprisone treated mice decreased the percentage of the brain pathological neurons. Following melatonin administration increase in NSC amount in the olfactory bulb of the hemiparkinsonic rats correlated with striatum antioxidant enzyme activities. Transplantation of the multipotent neuronal crest-derived stem cells (NCSCs) from bulbar region of the hair follicle into injury area of sciatic nerve results in significant increase of the distal nerve fibers. Animal models with pathology of the nervous system that were treated with LIF, melatonin or NCSCs had improved locomotor activity. The results of the research was presented in form of the report, 13 articles, 1 patent, 21 abstracts. Cuprisone, hemiparkinsonism, peripheral nerve, stem cells, hippocamp ischemia, regeneration Product Description popup.authors Васильєв Р.Г. Демидчук А.С. Кузьмінова І.А. Літошенко З.Л. Лабунець І.Ф. Мельник Н.А. Півнева Т.А. Подяченко О.В. Римар С.Ю. Родніченко А.Є. Утко Н.А. popup.nrat_date 2020-04-02 Close