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Information × Registration Number 0216U003916, 0115U005201 , R & D reports Title Obtaining сell lines f agricultural plants with ncreased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators. popup.stage_title Head Galkin Anatoliy Pavlovich, Registration Date 24-02-2016 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 The wheat plants of two varieties Yatran 60 and Zimoyarkawere introduced in vitro culture. The callus cells derived from hypocotyl and root segments of 5-day old wheat seedlings were obtained onnutrient media MS with bioregulatorsof microbial origin at concentrations 20-30 µl/l in combination with 1 mg/l auxin 2,4-D. The shoots and leaf buds were formed on MS nutrient medium with bioregulators at concentrations 10-50 µl/l with 1-2 mg/l cytokinin BAP. These shoots and leaf buds were transferred on MS medium with bioregulators for their development, growth, rooting, and further verification of their resistance to nematode in greenhouse conditions. The impact of bioregulators on increase (on 75-87%) wheat resistance to the parasitic nematode Heterodera avenaewas studied in the laboratoryconditions.The difference in the degree of homology (up to 37-65%) between cytoplasmic mRNA andsi/miRNA from cells of control and experimental plants was found using Dot-blot hybridization method.In the wheatsprouts cell-freesystemofproteinsynthesis the increase of silencing activity of si/miRNA from cells of experimental wheat plants (up to 48-78%) compared to the control was shown. Product Description popup.authors Іутинська Г.О. Білявська Л.О. Галкін А.П. Козирицька В.Є. Циганкова В.А. Шиша О.М. popup.nrat_date 2020-04-02 Close