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Information × Registration Number 0216U005004, 0115U003649 , R & D reports Title Implementation into clinical practice of immunodiagnostic test systems for the quantitative determination of a threat of thrombus formation for different diseases. popup.stage_title Head Lugovskoi E. V., Registration Date 11-01-2016 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 Cardiovascular diseases and surgeries accompanied by abnormal activation of the coagulation system, leading to the formation of a blood clot inside the vessel. During the execution of an analysis on sequence of tests for the diagnosis of disorders of hemostasis. Revealed that tests APTT (activated partial thromboplastin time) and PT (prothrombin time) does not allow us to determine the degree of activation of blood coagulation in a number of pathologies. It was established that the use of the developed test kits for quantitative determination of soluble fibrin and D-dimer in human blood plasma is within correctly assess the state of hemostasis. Optimized methods for preparing calibrators lyophilized proteins (fibrin and D-dimer) for the construction of calibration curves in diagnosing the threat of thrombosis. It was established that the maximum treating fibrin-specific monoclonal antibody I-3c used in the test system for the quantitative determination of soluble fibrin in blood plasma, from the culture medium occurs during affinity chromatography on fibrin-sepharose. On the basis of JSC SPC "Diaproph-Med" produced pilot batch of test systems "DIA-D-dimer", which was submitted for testing in clinical laboratories Kiev regional hospital Kharkiv Regional Hospital and Diagnostic Centre Medical Academy (c. Dnepropetrovsk). Product Description popup.authors Ганова Лариса Олександрівна Гоголінська Генріета Казимирівна Дубовецький Артем Сергійович Колеснікова Ірина Миколаївна Комісаренко Сергій Васильович Костюченко Олена Петрівна Наврулін Віктор Олегович Співак Микола Якович Урвант Леся Петрівна Хаджинова Вероніка Євгенівна popup.nrat_date 2020-04-02 Close