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Registration Number

0216U005160, 0111U000536 , R & D reports

Title

To work out the methods of introduction into the culture in vitro of ovules of sweet sorghum in oder to obtain dihaploid lines

Head

Bekh Natalya,

Registration Date

19-01-2016

Organization

Institute of a sugar-beet

Description

A study to determine the experimental approaches using biotechnological methods that may be used to create new homozygous and polyploid forms of sweet sorghum. Developed sterilization conditions initial explants, haploinduction, a longer time between passaging and poliploidy shoots of sweet sorghum. Haploinduction from unfertilized seed germs of sweet sorghum stimulates presence in the culture medium drop of 1 mg/l, glutamine 1 mg/l, BAP 0.5-5 mg/l. Improved cytological method of determining the level of the genome on metaphase plates the points of growth of cultural shoots of this plant. The resulting haploid breeding clones in four samples of sweet sorghum hybrids "Favorite", "Medoviy" and varieties of "Silosne", "Nectarniy." As a result of research installed efficiency activated carbon at a concentration of 250 mg/l in the medium for cultivating a longer time between passaging shoots sweet sorghum, by improving the growth process and prolong the viability of explants in culture in vitro to 4 months. The method of adaptation provides a high survival rate in soil polyploid plants.

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Бех Н.С.
Коцар М.О.
Щербіна І.В.