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Information × Registration Number 0216U005288, 0115U002698 , R & D reports Title Developing of prognostic markers for the assessment of the spermatogenic epithelium sperm-producing function under the ionizing irradiation popup.stage_title Head Andreychenko Sergiy Vadymovych, Registration Date 21-01-2016 Organization State institution "National research centеr for radiation medicine of the National academy of medical sciences of Ukraine " popup.description2 Object - blood, sperm and testis of laboratory anymals (rat). Aim - clarification of the ionizing radiation influence in different doses on spermatogenesis and rat male gonads hormone produced function for determination of the most radiosensitive biomarkers which characterized the state of generative epithelium at total and local irradiation of the rat . Methods - radiobiological, microscopic, spectrophotometric, morphologic, ELISA and statistical techniques. Dynamics of quantitative changes in colonization of seminiferous tubules of laboratory rats by both somatic Sertoli cells and different types of germ cells, in dependence from dose of total and local (testicules) gamma-irradiation and duration of post-irradiation period was revealed. It was shown that radiation practically does not affect number of Sertoli cells in seminiferous tubules, but in doses 1-2 Gy leads to death of differentiating A-spermatogonia and their number diminution by 60-80% in comparison with control. Due to higher radioresistance of spermatocytes, spermatids and sperms their radiation-induced damage appeared in doses 4 and 7 Gy. Doses 1 Gy and 2 Gy had practically no effect on sperm production quantity, leading only to morphologic anomalies in spermatozoa structure appearance. Doses 4 Gy and 7 Gy led to significant spermatogenesis suppression in association with suppression of viable spermatozoa production. Spermatogenesis suppression was reflected by down-regulation of synthesis of testosterone, inhibin B, anti-Muller hormone and, in contrast, with up-regulation of FSH, LH and estradiol in animal blood plasma. Product Description popup.authors Андрейченко Катерина Сергіївна Булавицька Вероніка Михайлівна Ватліцова Ольга Станіславівна Горбань Леся Вікторівна Клепко Алла Володимирівна Кондратова Юлія Анатоліївна Мотрина Оксана Анатоліївна Пчеловська Світлана Анатоліївна Саковська Леся Василівна Чернишов Андрій Вікторович popup.nrat_date 2020-04-02 Close