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Information × Registration Number 0216U006211, 0111U006156 , R & D reports Title To ground theoretically and elaborate technologies of obtaining and utilization of various types of cells for the efficient use of genetic resources in the embryonal biotechnology popup.stage_title Head Gevkan Ivan Ivanovych, Registration Date 16-02-2016 Organization Institute of animal biology Ukrainian academy of agrarion popup.description2 The effective method for obtaining a culture of granulosa and cumulus rabbits cell, studying the dynamics of proliferative activity and steroidogenesis at different initial concentrations of granulosa cells, optimal conditions co-cultivation of granulosa cells with of rabbit's oocytes, the influence of the electromagnetic field of microwave on maturation OCC to metaphase-2 during co-cultivation with culture of granulosa cell were studied. The effective method of obtaining culture of rabbits cumulus cells, studying the dynamics of their proliferative activity and the level of steroidogenesis with addition of various growth factors in the environment for cultivation and establishment of impact co-cultivation of cumulus cells and oocyte during its maturation to metaphase-2 were studied. The conception of creating a combined spatially organized cellular system with hyaluronic acid to improve the quality of gametes and embryos based on: learning intensity proliferative growth culture granulosa cells with hyaluronic acid index of proliferation, viability of the ability to synthesize cholesterol and steroid hormones, active enzymes of anaerobic respiration and maturation of oocytes cumulus complexes rabbits to metaphase-2 were done. A practical application creation combined spatially organized cellular system granulosa with hyaluronic acid and different concentrations of insulin and silver nanoparticles to improve the quality of gametes and embryos based on: learning intensity proliferative growth culture granulosa cells with hyaluronic acid index of proliferation, functional activity of the ability to synthesize cholesterol and steroid hormones, lactate dehydrogenase enzyme activity and maturation of rabbits' oocytes cumulus complexes to metaphase-2 and embryos to the transferable stage were showed. Product Description popup.authors Сирватка В. Я. Сливчук Ю. І. Штапенко О. В. popup.nrat_date 2020-04-02 Close