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Information × Registration Number 0216U006713, 0113U003764 , R & D reports Title The investigation of expresion of marker proteins epithelial stem cells and progenitor cells in oral squamous cell carcinoma. popup.stage_title Head Sydorenko Myhail V., Registration Date 28-04-2016 Organization Department of Biotechnical Problems of Diagnostics, Institute of Problems of Cryobiology and Cryomedicine of NAS of Ukraine popup.description2 Abstract. Research report: p.37, Figure-11, Table-5, literature sources-9. The object of study: MCF-7 cells, cryoprotective agents. Objective: Test Vial proposed "Nunc", Germany, for the determination of temperature gradients on the border crossings supercooled nitrogen-inner surface of the biological object, the container and the composition of cryoprotectants, to study the effectiveness of cell survival of MCF-7 and HT29 cells in a medium with modified cryoprotectants. Research Methods and apparatus: cryoprotective solutions (CS) consisted of the culture medium, serum, DMSO, sucrose, glycerol, ethylene glycol, polyethylene glycol, carboxymethyl cellulose (CMC) in different ratios. When you add the CS using five different protocols at a slow and super-quick freezing apparatus using mock sample. The results and their novelty: It is revealed correlative relationship between the molecular weight (MW) of the variable component of cryoprotectants and rate of temperature reduction in the Viale. The sign of the correlation coefficient between MW cryoprotectant and temperature lowering rate in the range of 0 ...- 40° C positive (r = 0, 8144), whereas in the range of -40 ...- 170° C - negative (r = -0,9096) . These survival using the MTT assay also indicate a strong negative correlation with the size of cryoprotectant molecules in the range of 342-20000 Dalton, r = -0,9908. Survival Index (MTT test) most cryoprotectants increased by adding CMC. Keywords: MTT-test, the metabolic activity of the cells, freezing, DMSO, sucrose, glycerol, ethylene glycol, polyethylene glycol, CMC.5481 Product Description popup.authors Бурлака Дмитро Петрович Сидоренко Михайло Васильвич Якимчук О.М. popup.nrat_date 2020-04-02 Close