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Registration Number

0216U006829, 0113U002112 , R & D reports

Title

To study the features of physiology, biochemical and immunological reactions at development of pain syndrome for animals different age

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Head

Bezrukov Vladislav Viktorovich, Butenko Gennadij Michajlovich,

Registration Date

16-06-2016

Organization

State Institution "Institute of Gerontology AMS Ukraine"

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It was shown in experiments on rats and miсe that under the design of nociceptive pain ("formalin test") the pain reactions in old animals appear later and are expressed in lesser degree that in adult individuals. At the conditions of chronic pain the substantial age-dependent changes of the cardiovascular system components were also revealed. After 1 month of sciatic nerve bandaging the sensitiveness of vascular wall to the vasoconstrictor action of noradrenaline changed in age-dependent ways. Whereas in adult rats the noradrenaline-induced constriction diminishes as compared to control, the corresponding reaction in old animals increases. In experiments on the isolated heart under chronic pain conditions the substantial age-dependent distinctions of myocardium retractive function in old rats as compared to adult animals were also shown. In conditions of experimental nociceptive pain the substantial age-related changes of immunological indexes characteristics were revealed. Whereas in adult mice the indexes of whole body mass, relative mass of thymus and cells number did not change under chronic pain conditions, in old animals the relative mass of thymus and the number of cells increased. The value of splenic index considerably increased in adult experimental mice, and, on the contrary, decreased in old animals. The studies of of peritoneal macrophages have shown that in old mice the loosening in the whole tissue phagocyte activity is due to the reduction of number of phagocyte macrophages, whereas in old individuals the corresponding tissue reaction was reasoned both by the reduction of incurrence of peritoneal and phagocyte macrophages and overall phagocyte number as well.Under the action of chronic pain in rats, we observed changes in peripheral blood. Thus, in adult rats was observed increase in the total number of leukocytes and relative content of neutrophils segmentojadernye. Old rats showed a decreasing relative amount of packaging of neutrophils and lymphocytes and growth of segmentojadernye relative content of neutrophils. In addition, under the influence of chronic pain in adult and older animals decreased primary immune response to sheep red blood cells The study of behavioral responses in the test "open field" and elevated cruciform maze showed that chronic pain leads to increased motor activity, expressed in greater extent in older animals, which indicates an increase in the level of anxiety and aggressiveness.

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Берук Ольга Олександрівна
Кирик Віталій Михайлович
Лабунець Ірина Федорівна
Олар Віталій Васильйович
Парамонова Галина Іванівна
Песчана Єкатерина Олегівна
Родниченко Анжела Євгенівна
Сикало Ніна Володимирівна
Холін Віктор Олександрович

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2020-04-02