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Information × Registration Number 0216U007476, 0115U002934 , R & D reports Title Testing of carbon-protein constructs for targeted delivery of anticancer drugs. popup.stage_title Head Sydorenko Myhail V., Registration Date 19-12-2016 Organization Department of Biotechnical Problems of Diagnostics, Institute of Problems of Cryobiology and Cryomedicine of NAS of Ukraine popup.description2 Abstract. Research report: 29 p., 6 fig., 1 photo, 1 tab., 32 sources of references. The object of study - tumor cell lines of hepatocellular carcinoma and adenocarcinoma of the breast, ultra-structural carbon and diamond carbon lukopodobny. The goal - to study the prospects for the use of carbon materials (diamond-like carbon and lukopodobnogo) as carriers of anticancer drugs for targeted delivery. Research methods - methods of cell culture, cyto-morphological, MTT test, microscopy. As a result of the second phase of the research work demonstrated the effectiveness of trypsin as a release agent MLC particle UDD/OPS-DOK; UDD particles and /OPS-DOK as a carrier for drug delivery. The paper showed that the trypsin-mediated release of PKD is dose-dependent and a negative correlation with tumor cell viability. Survival adenocarcinoma cells (MCF-7), when incubated with UDA-MLC for 24 hours decreased from 52 to 28% (UDD concentration from 8.4 to 670 ug/ml MLC - from 2.5 to 20 micrograms/ml) and when incubated with OPS-PKD at the same concentrations and reaction time - from 72 to 30%. Hepatocellular carcinoma cell sensitivity to MLC was higher than that of MCF-7 cells. Thus, based on these results, we suggest that the carbon-based protein complex UDD APs and loaded with anticancer drugs and specific antibodies can be useful for the effective delivery of an anticancer agent. Keywords: Tumor cells are hepatocellular carcinoma and breast cancer, ultra disperse nanodiamonds, carbon lukopodobny, adhesion, proliferation. Product Description popup.authors Гергелюк Тетяна Сергіївна Перепелиціна Олена Михайлівна Сидоренко Михайло Васильвич Ястребова Олена Вікторівна popup.nrat_date 2020-04-02 Close