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Information × Registration Number 0216U009196, 0115U004187 , R & D reports Title Using molecular and cellular technologies for biotech wheat and maize plants resistant to the herbicide glyphosate obtaining popup.stage_title Head Morgun Bogdan Volodymyrovych, Registration Date 30-12-2016 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 Primary explants for cereal Agrobacterium-mediated transformation were selected: 18 day callus cultures derived from apical meristems of aseptic 3-day wheat seedlings; 6-12-day callus cultures derived from immature wheat germ; prolonged cultivation culture of regenerative-active callus derived from immature embryos, or 6-12 day callus derived from immature maize embryos. The optimal culture conditions for wheat and maize regeneration were chosen. The effective elimination conditions of Agrobacterium cells are selected. The technique and conditions of maize and wheat Agrobacterium-mediated genetic transformation were elaborated. The effective selection technique of transformed plants was elaborated: paromomycin, phosphinothricin (glufosinate ammonium) and glyphosate (N- [phosphonomethyl] glycine) were used as selective agents. Product Description popup.authors Банникова Марія Олександрівна Горбатюк (Волч) Ірина Романівна Нітовська Ірина Олександрівна Тараненко Артем Миколайович popup.nrat_date 2020-04-02 Close