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Information × Registration Number 0216U009204, 0115U005409 , R & D reports Title Molecular biological effects and mechanisms of lactoferrin action on tumor cells in vitro and in vivo popup.stage_title Head Chekhun Vasyl Fedorovych, Registration Date 30-12-2016 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology popup.description2 It was found that after incubation of breast cancer cell lines with lactoferrin the increase the level of reactive oxygen species (in 1,4-1,8 times, p <0,05), inducible NO generation rate of NO-synthase (1,5-2 1 fold, p <0,05) and the level of "free iron" (1,7-2,5 times, p <0,05) in all studied lines was observed. Moreover, the effects of lactoferrin were more prominent in hormone-negative cell lines (MDA-MB-231, MDA-MB-468). These changes resulted in increased expression of proapoptotic protein Bax (by 13-17%, p<0,05), reducing in expression of antiapoptotic protein Bcl-2 (16-23%, p <0,05) and mitochondrial phospholipid cardiolipin (in 1.4-1.7 times, p <0,05) content. This, in turn, caused an increase in the number of cells died by apoptosis (by 14-24%, p <0,05). Cytotoxic effects of lactoferrin was accompanied by an increase in the percentage of DNA "comet tail" (3-4 fold, p <0,05) and blocking cell cycle at G2/M phase, especially in hormone-negative cell lines. Thus, a study found that lactoferrin causes a violation of pro/antioxidant balance by increasing the level of reactive oxygen species, "free iron" and speed of NO generation. These violations resulted in the blocking of cell cycle at G2 / M- phase and the death of malignant cells by apoptosis. The obtained data reveal fundamental perspective of lactoferrin usage in chemotherapy. Product Description popup.authors Задворний Тарас Володимирович Лук'янова Наталія Юріївна Лукін Сергій Миколайович Сторчай Дарія Миколаївна Трембач Людмила Валентинівна Чехун Василь Федорович Яловенко Тетяна Миколаївна popup.nrat_date 2020-04-02 Close