Search academic texts

Information × Registration Number 0216U009206, 0115U002933 , R & D reports Title Creation of new rhizobia strains resistant to unfavorable environmental conditions by biotechnological methods, to improve bacterial preparations popup.stage_title Head Kots Sergii Yaroslavovych, Registration Date 03-01-2017 Organization Institute of Plant Physiology and Genetics NASU popup.description2 The results of the work have shown that the frequency of occurrences of Kmr cells depended on both the genotype of nodule bacteria involved in Tn5 mutagenesis and the duration of the conjugation mixture of donor and recipient exposure. The frequency of occurrences in the strain 159 of R.galegae was 4,4·10-8 and 1,6· 10-7 and in the strain 0703 of R. galegae was 1,9·10-7 and 1,3·10-7 depending on the exposure time (6 and 18 hours) when using the vector pSUP5011::Tn5mob. Transposon mutagenesis involving the vector pSUP2021 caused the frequency of occurrences 3,1?10-9 and 2,4?10-9 in case of R.galegae 159 and 1,2?10-7 and 1,8?10-7 using the strain 0703 of R. galegae. The transposon mutagenesis of R. galegae 0702 was ineffective. It was shown that the strains B16 and B20 of B.japonicum created by the vector pSUP5011::Tn5mob had poor growth and EPS contents in the growth media compared to B.japonicum T21-2 (transposon mutant with plasmid pSUP2021::Tn5). The strain of alfalfa nodule bacteria S.meliloti AC88 selected by analytical methods synthesized high amount of EPS and had high growth activity compared to S.meliloti 425а. It was established that the contents of protein in bacterial cells depended on the genotype of rhizobia. This fact can be taken into account when new express markers of symbiosis efficacy are developed. Product Description popup.authors Береговенко Світлана Кирилівна Воробей Надія Анатоліївна Кондратюк Юлія Юріївна Коць Сергій Ярославович Мельникова Наталя Миколаївна Рибаченко Лілія Ігорівна popup.nrat_date 2020-04-02 Close