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Information × Registration Number 0216U010224, 0116U007060 , R & D reports Title Engineering secondary metabolism of streptomycetes using mutations in gene for ribosomal protein S12 popup.stage_title Head Ostash Iryna Stepanivna, Registration Date 23-12-2016 Organization Ivan Franko National University of Lviv popup.description2 A subject of research is mechanisms of coexistence of rpsL mutant alleles in Streptomyces cells and primary structure of S. cyanogenus S136 genome. The object of the study are rpsL gene merodiploids strains S. cyanogenus and S. albus J1074 and genome S. cyanogenus S136. The purpose of research is to study the effect of the mutant allele of the ribosomal protein S12 gene on the secondary metabolism of S136 and J1074. A streptomycin resistance of merodiploids S.cyanogenus and S. albus strains were investigated. A gene ORF 3375 was found in S136, genome of which possesses a high level of similarity with the product-of transporter landomycins gene lanJ, located in the lan-cluster. A system for detection of mutant S12 protein incorporation into ribosome was constructed. Product Description popup.authors Дацюк Юрій Ростиславович Осташ Богдан Омелянович Пиж Марія Василівна popup.nrat_date 2020-04-02 Close