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Information × Registration Number 0217U000968, 0112U000614 , R & D reports Title Changes in expression of genes and activity of enzymes involved in carbohydrate and energy metabolism in malignant cells as mean of tumor growth suppression popup.stage_title Head Sibirny Andriy Andriyovych, Registration Date 23-03-2017 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Influence of lipid-protein carbohydrates free diet, glibenclamide and metformin on some parameters of carbohydrate and energy metabolism of model Nemeth-Kellner lymphoma (NK/Ly) cells in female mice of Balb/c line was studied. It was shown that lymphoma cells of the animals of the control group (C) and those on the diet (group D), did not differ in the activity of pyruvatkinase. Administration of metformin or glibenclamide to the animals caused the decrease of the enzymatic activity. At the same time the activities of hexokinase and phosphofructokinase in the lymphoma cells of the animals of group D were lower compared to the control group. Concomitant administration of glibenclamide and metformin resulted in a significant decrease of the hexokinase activity and increase of the phosphofructokinase to the level of control. The respiratory activity of the lymphoma cells was on the level of sensitivity of the used device and did not significantly differ in the animals of the different groups. It was shown that the content of ATP in the lymphoma cells of the animals of group D was higher compared to the control, administration of glibenclamide and metformin to mice significantly reduced this parameter in lymphoma cells. It was found that the transfer of the animals on the diet and using of metformin caused a decrease of lactate concentration in the ascetic fluid of mice, whereas glibenclamide increased this parameter. The transfer of the mice on the carbohydrates free diet and administration of glibenclamide and metformin to them extended the life of animals by an average of 67%±25% and 63%±25% respectively.5481 Product Description popup.authors Борецький Юрій Романович Куриленко Олена Олександрівна Лизак Олексій Орестович Семків Марта Віталіївна Фаюра Любов Романівна Федорович Дарія Василівна Юрків Мар"яна Тарасівна popup.nrat_date 2020-04-02 Close