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Information × Registration Number 0217U002313, 0116U004864 , R & D reports Title The application of metabolic engineering approaches for construction of Saccharomyces cerevisiae strains able to overproduction of glycerol under anaerobic conditions. popup.stage_title Head Dmytruk Kostyantyn Vasylovych, Registration Date 03-02-2017 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 To obtain strains of yeast Saccharomyces cerevisiae with limited pyruvate decarboxylase activity (different isoforms of which are encoded by genes PDC1, PDC5 and PDC6), crossing between strains dpdc1 and dpdc5 S. cerevisiae was performed. Obtained recombinant S. cerevisiae strain with double deletion dpdc1 dpdc5 revealed practically no activity of pyruvate decarboxylase, was unable to grow on the glucose as a sole Carbon source, produced minor amounts of ethanol and increased amounts of glycerol in comparison with the wild-type strain. Introduction of heterologous genes ACL1 and ACL2, encoding two subunits of ATP-dependent citrate lyase Yarrowia lypolytica into the genome of S. cerevisiae dpdc1 dpdc5 strain restored its ability to grow on the glucose as a sole Carbon source but didn't influence much the glycerol production during alcoholic fermentation. Product Description popup.authors Дмитрук Костянтин Васильович Куриленко Олена Олександрівна Семків Марта Віталіївна Сибірний Андрій Андрійович popup.nrat_date 2020-04-02 Close