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Information × Registration Number 0217U002835, 0116U001218 , R & D reports Title Elaborate the system of complex estimation of the genofond of cultivated species of fish for improving the selection process. popup.stage_title Head Tarasjuk Sergey Ivanovich, Доктор сільськогосподарських наук Registration Date 12-12-2017 Organization Institute of Fisheries NAAS popup.description2 The conducted studies allowed to determine the factors that have the greatest impact on the amplification efficiency, namely: the concentration of the DNA preparation, the concentration of the primer in the reaction mixture and the number of amplification cycles. The most polymorphous was the locus MFW 23 (77,3 %), and the least polymorphic was the locus MFW 06 (64, 0 %). The effective number of alleles in the sample of genotypes studied varied from 2.14 (MFW 31) to 5.23 (MFW 23). According to calculations of allelic frequencies, the main indicators of genetic variability are determined. The maximum level of available heterozygosity is fixed for the MFW 23 locus, the lowest for the MFW locus 31. The results obtained for the use of the micronucleus test indicate that the chromosomal apparatus of the carp on the studied cytogenetic characteristics in the peripheral blood cells was more stable in the groups of scaly carp than in the frame. First of all, this is due to morphological features of the carpal carp. Under the influence of the same environmental factors on the hereditary apparatus of fish, carp species of the feminine breed, in comparison with scales, are characterized by a higher level of destabilization of the genetic apparatus. Product Description popup.authors Глушко Юлія Миколаївна Залоїло Ольга Василівна Маріуца Алла Ергашівна Постоєнко Д.М. Тарасюк Сергій Іванович popup.nrat_date 2020-04-02 Close