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Information × Registration Number 0217U002995, 0116U006170 , R & D reports Title Study of the role of purine and glutamate receptors of sensory neurons in pain signaling popup.stage_title Head Kulyk Vyacheslav Borisovich, Registration Date 28-12-2017 Organization Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine popup.description2 A comparison of the effect of endogenous leu-enkephalin opioid on ionic current mediated by P2X3 receptors in neurons of dorsal (DG) and nodоum (NG) ganglia. About 19% of NG cells express exclusively P2X3 receptors sensitive to the action of leu-enkephalin on membranes compared to DG neurons (25%). Like DG neurons, P2X3 receptors of NG neurons are reversibly inhibited by leu-enkephalin, depending on the concentration and time of application of the latter. In both types of neurons, the inhibitory effect of leu-enkephalin was eliminated using the inverse opioid receptor antagonist-naloxone. This indicates that the activation of opioid receptors by the endogenous opioid leu-enkephalin leads to inhibition of P2X3 receptors. However, the sensitivity of P2X3 currents to opioids in the NG neurons was sharply different in comparison with DG. Thus, half the effective concentration (IC50) in the NG neurons was 1 ?m, whereas in DG this index was about 10 nM. Previous incubation of DG neurons with naloxone led to an increase in the inhibitory effect of leu-enkephalin and changed the IC50 from 10 nM to 1 nM. In contrast, the preincubation of cultured neurons of NG with naloxone did not cause an increased inhibitory effect of opioids on P2X3 receptors. This may indicate the involvement of various molecular pathways that mediate the relationship between opioid and P2X3 receptors in DG and NG neurons. Product Description popup.authors Кулик В.Б. Шипшина М.С popup.nrat_date 2020-04-02 Close