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Information × Registration Number 0217U003018, 0116U004299 , R & D reports Title Nicotinic acetylcholine receptors, which support differentiation and viability of the brain cells. popup.stage_title Head Skok Maryna Volodymyrivna, Доктор біологічних наук Registration Date 13-01-2017 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 The study deals with the role of nicotinic acetylcholine receptors (nAChRs) expressed in mitochondria and in pluripotent stem cells capable to differentiate into brain cells. It is found that in addition to alpha3beta2, alpha4beta2 and alpha7beta2 nAChRs revealed previously, mitochondria contain also alpha9 nAChRs, their content being increased significantly in the absence of alpha3, alpha7 and/or beta2 subunits. It is shown that allosteric changes in the nAChR molecule are sufficient to activate signaling cascades necessary to protect mitochondria from activation of apoptosis pathway. The data obtained allow stating that efficient anti-apoptotic protection is realized through combined action of all mitochondrial nAChRs expressed and connected to different kinase cascades. Nicotinic receptors of at least three subtypes, alpha3beta2, alpha7 and alpha9, were found in mesenchymal stem cells of human and mouse and in human induced pluripotent stem cells. Their expression is being changed in the course of cell cultivation in vitro or cryoconservation and is maintained along with differentiation into neurons. alpha7 nAChRs regulate the production of pro-inflammatory interleukin-6 by mesenchymal stem cells subjected the bacterial lipopolysaccharide. Product Description popup.authors Коваль Людмила Миколаївна Лихмус Олена Юріївна Успенська Катерина Романівна popup.nrat_date 2020-04-02 Close