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Information × Registration Number 0217U004067, 0116U007715 , R & D reports Title Photosensitivity of Nanostructured Bioactive Molecular Systems popup.stage_title Head Bulavin Leonid Anatolievich, Registration Date 13-01-2017 Organization Taras Shevchenko Kiev University popup.description2 An object of the study - ceftriaxone, copper nanoparticles, N-acetylglucosamine (hydrocarbon component of the bacterial cell membranes), phosphatidylcholine (lipid component), D-alanine, D-glutamic acid, L-lysine, L-cysteine (protein component) Subject of investigation - restructuring of electronic states; optical, quantum-chemical and therapeutic properties of copper nanoparticles with ceftriaxone. Objective: Optimizing the therapeutic efficacy of ceftriaxone with copper nanoparticles, its physicochemical and biological properties with new highly sensitive optical techniques, such as giant surface-reinforced Raman scattering (SERS), fluorescence, methods of atomic force spectroscopy and bionanomechanics for detecting supersmall amount of chemical and biological molecules, fluorescent imaging, studying the restructuring of electronic states in solutions of copper nanoparticles and antimicrobial drug ceftriaxone with bacterial cell membrane components, namely N-acetylglucosamine (hydrocarbon component of the bacterial cell membrane), phosphatidylcholine (lipid component ), D-alanine, D-glutamic acid, L-lysine, L-cysteine ( protein component) to determine the changes in the interactions of nanomaterials substance compared to the original antibiotic. Research Methods: Surface-enhanced Raman scattering (SERS) with sub-micron resolution, infrared spectroscopy, optical absorption, photoluminescence microspecroscopy, atomic force and electron microscopy, X-ray analysis. Research results: 1. Basic 0-0 'shift in the absorption spectrum of ceftriaxone defined by the basic configuration HOMO > LUMO + 1 orbitals which have p-type localized on benzene rings. The second major transition due to the configuration of HOMO-3>LUMO, belonging to the p-type orbitals localized on a saturated carbon chain molecules of ceftriaxone. 2. Ther is a low-intensity absorption band at a wavelength of 500 nm in the absorption spectrum of copper nanoparticles, which corresponds to the surface plasmon resonance, which is typical for copper nanoparticles. 3. The absorption spectra of ceftriaxone and its conjugate with copper nanoparticles are not additive, that is indicating the interaction between its components. 4. The consequence of establishing the interaction between copper nanoparticles with ceftriaxone molecules in the conjugate is the emergence of antibiotics enhanced action on protein, lipid, hydrocarbon components of the bacterial cell membrane, which manifests itself in the microbicides electron states reconstruction, that leads to changes in position and half-width of strips dipole allowed optical transitions. 5. The study of the samples by infrared spectroscopy showed a shift of the absorption bands of ceftriaxone functional groups: carboxylate, beta-lactam, amide - which can be a sign of change in anions coordination bonds of ceftriaxone with the glycine amino acid. Keywords: ceftriaxone, copper nanoparticles, components of the bacterial cell membrane, SERS Product Description popup.authors Гапонов Антон Миколайович Гончаренко Надія Анатоліївна Дмитренко Оксана Петрівна Ковальова Дар'я Олександрівна Куліш Микола Полікарпович Павленко Олена Леонідівна Стрельчук Віктор Васильович popup.nrat_date 2020-04-02 Close