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Information × Registration Number 0217U004255, 0116U007224 , R & D reports Title Studying the molecular properties of the virus Sharkey plums in Austria and Ukraine and development of biotechnological methods to produce virus-free planting material. popup.stage_title Head Polyschuk Valeriy, Registration Date 27-01-2017 Organization Taras Shevchenko Kiev University popup.description2 The object of study - the virus Sharkey plums (VSHS) Objective - monitoring nadsadzhen stone fruit crops in staggered VSHS; shtamovoyi accessories and definition of phylogenetic analysis to establish the phylogenetic relationship between Ukrainian isolates VSHS establish their origin, so that the development plan spread of the virus in different regions of Ukraine and neighboring countries, to predict the development of possible epidemics caused by more aggressive strains. Methods: The visual diagnosis, ELISA, methods of isolation of total RNA modification of polymerase chain reaction (PCR) analysis filohenetchnyy, technology clonal micropropagation of virus-free planting material of stone fruit plants. Using different versions of PCR for diagnosis VSHS in samples of stone fruit crop collected from industrial plantations Kyiv, Cherkasy and Odessa regions, found D and M strains of the virus to Sharkey plum apricot, cherry, plum, peach and plum. Using phylogenetic analysis conducted comparative characterization of isolates VSHS of Ukrainian deposited in the archives database GenBank. According to the results of phylogenetic analysis of gene capsid protein VSHS found that isolates VSHS of Odessa area most related to strains VSHS from the US and Germany, isolate from the Kiev region - with strains from the United States and Canada, isolate from Cherkasy region most related to strains VSHS from Belarus, Poland and Germany. In the study of stamps VSHS circulating in Austria, found that the most common strain of D. In some regions there is a strain of M. revealed similarities Ukrainian and Austrian isolates. To ensure a high level of reliability out virus-free planting material proposed model system that improves plant productivity and economic profitability of the industry. Product Description popup.authors І.Г. Будзанівська В.П. Поліщук С.М. Петренко popup.nrat_date 2020-04-02 Close