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Information × Registration Number 0217U004344, 0116U002472 , R & D reports Title Morphological changes in the cell culture of the thyroid gland of irradiated rats popup.stage_title Head Lavrenchuk Halina, Registration Date 31-01-2017 Organization State institution "National research centеr for radiation medicine of the National academy of medical sciences of Ukraine " popup.description2 Objective: To investigate morphological changes in the primary cell culture of rat thyroid infants whose parents were prenatally exposed radioisotope iodine-131. The object of study: biological effects in cell culture thyroid newborn rats prenatally exposed radioisotope iodine-131. Research Methods: obtaining and culturing the primary cell cultures of thyroid tissue of newborn rats, cytological (receipt and analysis of cell cultures agents for optical microscopy), biophysical (flow cytometry) statistics. An initial culture thyroid newborn rats and investigated the structural and morphological characteristics. It is shown that the intact cultures thyrocytes formed uniform continuous monolayer of cells with round nuclei and stratification similar cells that resembled functioning "follicle" thyroid tissue, cavities are filled with colloid. Analysis and quantitative morphological parameters of cells in primary culture thyroid newborn rats prenatally exposed radioisotopes of iodine-131 (1 generation) showed a tendency to increase the number of cells with micronuclei and dual-cells, which may be indirect evidence of instability of the genome of cells of the thyroid gland. It is shown that in cell culture thyroid newborn rats whose parents were exposed in utero (2nd generation), structural and morphological changes depend on who the parent was irradiated. Significant destructive symptoms was observed in cell cultures of rat thyroid exposure in option females and both parents density cell population reduced in 5.6 times (compared to control), and the amount of dual cells and cells with micronuclei increased 6-8 times. Number of apoptotic cells in cultures studied thyrocytes increased 25 times (compared to control) in the case of exposure of both parents and 11 times the exposure in the case of females only. Established that prenatal exposure of males has less influence on the formation of structural and morphological abnormalities in rats culture thyrocytes 2nd generation. However, they were found increasing the number of polyploid cells and cells with micronuclei 3.5 times compared to the control. The number of cells in various stages of apoptosis increased 7 times compared with the control. In cultures of rat thyrocytes 2nd generation was found "annular" cells, which are the basis of neoplastic transformation and evidence of significant violations and genotoxic genome instability in offspring of animals exposed radioisotope iodine-131. Product Description popup.authors Бойко Оксана Анатоліївна Гапєєнко Дар'я Дмитрівна Козловська Ірина Володимирівна Литвинчук Христина Михайлівна Оксамитний Віктор Миколайович Олейнікова Тетяна Георгіївна popup.nrat_date 2020-04-02 Close