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Information × Registration Number 0217U006345, 0114U001383 , R & D reports Title Investigation of polymorphism of receptors that determine the molecular pattern associated with pathogens and species spectrum of pathogens of some sex transmitted infections in Ukraine popup.stage_title Head Bilozorov Olexiy Pavlovich, Registration Date 29-03-2017 Organization Ukranian research institute of dermatology and venerology popup.description2 The object of study - the polymorphism of receptors that determine the molecular pattern associated with pathogens. The purpose of research - to study the polymorphism of receptors that determine the molecular pattern associated with pathogens and species spectrum of pathogens of some sex transmitted infections. Research methods - clinical, microbiological, serological, immunological, molecular biological, statistical. The research of polymorphism of receptor genes determining the molecular patterns of pathogens, - the first exon of the gene mannan-binding protein MBL2-G230A, gene toll-like receptors TLR1-I602S and gene receptor antagonist IL-1, IL-1RN 86-bp VNTR in patients with urogenital pathology was done. The data on the distribution of alleles in Ukrainian population was obtained. The association of polymorphisms of receptors that determine the molecular patterns of pathogens, with the features of the urogenital pathology was researched. It is shown that in men - low-activity allele carriers S polymorphism I602S TLR1 gene increased the likelihood of complicated urogenital diseases with the development of prostatitis, epididymitis, orchitis. Carriers active I allele are more likely easy for urogenital disease, which manifests urethritis in men and endocervicitis in women. The results indicate that the urogenital pathology is largely dependent on the activity of an important receptor - toll-like receptor 1 revealed decline in phagocytic index and serum opsonizing activity allele carriers 0 (A) gene MBL2-G230A, which allows to identify the group increased risk of morbidity infectious diseases. The research of characteristic features of the modern flow of chlamydial urogenital infection. The tendency to reduce the level of infection C.trachomatis, which reached a low in 2015 and changed to increase in 2016. To rule out as possible reasons of this tendency the mutation of cryptic plasmid or inhibition of DNA polymerase the studies was provided. It is shown that in the diagnosis of chlamydial infection should in accordance with current recommendations focus on the results obtained by the methods of nucleic acid amplification, rather than serological and immunofluorescence methods. It is shown that the most characteristic feature of modern epidemiology and clinical manifestations of syphilis is the prevalence of late forms of the disease. When determining the molecular markers of resistance to macrolides T.pallidum - nucleotide substitutions in the gene 23S rRNA has not been detected T.pallidum strain resistant to macrolides. E.coli strains obtained by expressing a recombinant outer membrane protein of Chlamydia trachomatis MOMP in pRSETa vector. The strain of the microorganism Escherichia coli - producing recombinant protein of the outer membrane of Treponema pallidum pRSET/Tp-Porin. Product Description popup.authors Білозоров Олексій Павлович Білоконь Ольга Миколаївна Бондаренко Глеб Михайлович Волкославська Валентина Миколаївна Гончаренко Валентина Василівна Григорова Ріца Миколаївна Губіна-Вакулик Галіна Іванівна Губенко Тетяна Володимирівна Гутнєв Олександр Леонидович Кутова Валентина Василівна Мілютіна Олена Йосипівна Маштакова Ірина Олексіївна Мещерякова Наталя Віталіївна Нікітенко Інна Миколаївна Пугачова Оксана Вікторівна Сокол Оксана Анатоліївна Стрижак Катерина Гнатівна Унучко Сергій Васильович Частій Тетяна Володимирівна Щоголєва Олена Володимирівна popup.nrat_date 2020-04-02 Close