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Information × Registration Number 0217U006424, 0112U002174 , R & D reports Title Mechanisms of in vitro and in vivo effects of novel compounds possessing proapoptotic activity, the ways of enchancing their antineoplastic action and grouding new approaches in diagnostics and estimation of development of autoimmune and oncohematological diseases popup.stage_title Head Stoika Rostyslav, Registration Date 07-06-2017 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Molecular mechanisms of antineoplastic activity of new antibiotics of the family of landomomycins have been studied. The role of a mitochondrial pathway of apoptosis induction by landomycins of various length of its carbohydrate moiety and the presence of hydroxyl-containing group in the 11-position of aglycone landomycin was studied. The investigation of biochemical mechanisms of in vitro and in vivo antitutor action of traditional (Doxorubicin and Cisplatin) and experimental (4-thiazolidinone) antitumor substances at their delivery in a target cell by the nanosized carriers based on fullerene C60 and new comb polymeric carriers containing polyethylene glycol "was conducted. It was shown that doxorubicin immobilized on a polymeric nanoscale carrier poly (VEP-GMA) -graft-PEG reduced the relative amount of in vitro growth of mammalian cancer cells at 10 times lower dose than free doxorubicin. A decrease in the manifestations of hepatotoxicity (activity of alanine and aspartate aminotransferase in blood serum of mice with NK/Ly lymphoma and acute leukemia L1210) and cardiotoxicity (change in the distribution of isoenzymes of lactate dehydrogenase in blood serum of tumor-bearing mice) of doxorubicin after its delivery by the synthesied polymeric carrier has been established. The parameters of cardio-, hepato- and nephrotoxicity in blood serum of rats injected with 4-thiazolidinonium (ID-3833, ID-3288, ID-3882) were introduced without and after encapsulation on polymeric nanosized carriers. The normalization of these indicators was established when introducing complexes in comparison with the indices obtained with the use of unencapsulated compounds. The enhanced cytotoxic actions of cisplatin and its enhanced ability to overcome acquired resistance of tumor cells to chemotherapeutics cisplatin by immobilizing on a fullerene C60 - nanoscale carrier was demonstrated. The role of desialization of the cell surface in intercellular interactions has been established, in particular, the trans-sialing of the surface of the neighboring cells has been experimentally proven, as well as the effect of desialization of the cell surface on the processes of phagocytosis and efferocytosis. The molecular mechanisms that can mediate the negative regulation of the Wnt signaling pathway by the protein product of the PTTG1 gene, securin, are determined. It has been established that the temporal overexpression of the PTTG1-binding factor (PBF1) results in the blocking of early BMD-induced osteogenesis. At the same time, temporal overexpression of hairpin-forming RNAs (shRNA) that block the expression of PTTG1 and PTTG1-binding factor (PBF1) resulted in stimulation of early BMD-induced osteogenesis. Product Description popup.authors Антонюк Володимир Олександрович Білий Ростислав Олександрович Бойко Наталя Миколаївна Думич Тетяна Ігорівна Кіт Юрій Ярославович Ключівська Ольга Юріївна Корчинський Олександр Генадійович Легка Ліля Володимирівна Луцик Максим Дмитрович Магорівська Ірина Богданівна Малишева Христина Володимирівна Панчук Ростислав Русланович Сеньків Юлія Василівна Скорохід Надія Романівна Старикович Марина Олексіївна Стойка Ростислав Степанович Фінюк Наталя Степанівна Чумак Віра Володимирівна Шафранська Галина Іванівна popup.nrat_date 2020-04-02 Close