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Information × Registration Number 0217U006913, 0113U002494 , R & D reports Title Glutamate-sensitive biosensor: optimization, trial operation, development of strategy and methodology for the analysis of active glutamate transport in brain nerve terminals and platelets as a peripheral model of presynapse in norm and neuropathology. popup.stage_title Head Borisova Tatiana Alexandrovna, Registration Date 22-12-2017 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 An amperometric biosensor was developed and the concentration of glutamate and the effect of the working solution composition on its analytical characteristics was investigated. New methodical approaches for estimating the dynamics of accumulation of glutamate and stimulated depolarization of the plasma membrane of transporter-dependent release of glutamate from the nerve terminals of the brain using biosensor have been developed. The presence of the base signal in the preparation of the isolated nerve terminals of the brain in the absence of exogenous glutamate is shown. A methodical approach is developed for estimating the dynamics of tonic release of glutamate from nerve terminals, which is significantly increased under conditions of hypoxia and cholesterol deficiency in nerve terminals and causes the development of neurotoxicity under these conditions. The distribution of endogenous glutamate between the extracellular space and the cytoplasm of the nerve terminals is determined. The development of a method for the use of a glutamate-sensitive biosensor for the analysis of active transport of glutamate in nerve terminals of the brain will allow the use of this sensor in medicine, as well as in biochemical and biotechnological research. Product Description popup.authors Борисов А.А. Дударенко М.В. Крисанова Н.В. Кучеренко І.С. Назарова А.Г. Пастухов А.О. Позднякова Н.Г. Солдаткін О.О. popup.nrat_date 2020-04-02 Close