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Information × Registration Number 0217U006952, 0117U003381 , R & D reports Title Regenerative effects at coculturing of different origins stem cells with hippocampal slices after modeling ischemic injury popup.stage_title Head Rybachuk Oksana, Registration Date 27-12-2017 Organization Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine popup.description2 Studied regenerative effects of different origins stem cells: multipotent mesenchymal stem cells of adipose tissue (MMSC-AT) and multipotent stem cells of nerve crest derivatives (MSC-NC) in the in vitro model of short-term ischemic damage of hippocampus by immunohistochemical method. Ischemic injury was modeled by 10-minute oxygen-glucose deprivation. Two hours after modeling of ischemic damage GFP-positive stem cells - MMSC-AT and MSC-NC, - were applied on cultivated slices. The integration and differentiation of GFP-positive cells in recipient tissue in different periods after their application was investigated by means of immunohistochemical method using laser scanning confocal microscope. It has been shown that GFP-positive cells kept their vitality after transplantation on organotypic slices, embedded in the hypocampal neuropil, and MMSC-AT differentiated into NeuN-positive cells. Product Description popup.authors Забенько Є.Ю. Рибачук О.А. popup.nrat_date 2020-04-02 Close