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Information × Registration Number 0217U007427, 0117U006088 , R & D reports Title Diagnosis of wheat root diseases popup.stage_title Head Kriuchkova Larysa Oleksijivna, Registration Date 04-01-2018 Organization National university of Life and Environmental Sciences of Ukraine popup.description2 The object of study is the techniques of root disease diagnosis and identification of pathogens. Aim of research - isolation and identification of Gaeumannomyces spp based on cultural and morphological characteristics and pathogenicity factor, testing of set high specific primers on DNA from these isolates for future development of molecular test-system for diagnosis take-all and Fusarium root rot of wheat. Methods of research - field (sampling of winter wheat plants and visual diagnosis of their diseases), laboratory ( isolation of fungal pathogens in pure culture, study of their cultural and morphological characteristics, screening isolates presumably identificated as G.graminis), green-house experiments (artificial inoculation, pathogenicity identification and confirmation plant in vivo), molecular-genetic (design and analyze in BLAST new sets of specific primers for PCR identification species and variants Gaeumannomyces and their testing on DNA from isolates Gaeumnnomyces). The importance of results is the creation and confirmation of high-level specificity of primers complementary to ribosomal claster of DNA on the isolates of G.graminis. Using these primers the amplicons of 474, 400 and 591 bp were revealed in fungal culture, which correspond to variants of G.graminis var. avenae, G.graminis var.tritici and species G.graminis. The results of molecular analyzes not only confirm the occurrence of take-all on winter wheat in farms where the samples were taken. Since, only using molecular diagnosis methods the variant G.graminis var.avenae characterized as a highly virulent was detected on the wheat samples this is an evidence that the molecular methods of root disease of winter wheat sould be introduced into practice of phytosanitary monitoring of cereals crops.5481 Product Description popup.authors Антоненко Олексій Федорович Кирик Микола Миколайович Крючкова Лариса Олексіївна Піковський Мирослав Йосипович popup.nrat_date 2020-04-02 Close