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Information × Registration Number 0217U007436, 0115U001403 , R & D reports Title Establishment and characterization of a cell culture models based cultivation of tumor and stromal cells for estimation of effectivity antitumor drugs popup.stage_title Head Filonenko Valeriy Viktorovitch, Доктор біологічних наук Registration Date 05-01-2018 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 During the implementation of the third stage of the project, a method for assessing the migration ability of tumor cells in the MCF-7 line during the transformation of a multicellular spheroid into a cell monolayer colony was developed by measuring the change in the area of the migrating aggregate. The migration activity of the cells is proposed to be evaluated as the dynamics of the increase in the radius of the rolled cell colony in time. It is determined that the highest migration activity is inherent in cells for 2 days and significantly decreases by the third. The proposed model was tested on spheroids exposed to rapamycin (the mTOR / S6K signaling pathway) and its significant depressant effect on locomotor properties of MCF-7 cells was confirmed. The obtained results indicate the suitability of this model for estimating cell mobility.5481 Product Description popup.authors Хоруженко Антоніна Іванівна popup.nrat_date 2020-04-02 Close