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Information × Registration Number 0218U000001, 0117U006124 , R & D reports Title Role of epithelial-mesenchymal transition in mechanisms of drug resistance formation in prostate cancer cells popup.stage_title Head Lykhova Olexandra Olexandrivna, Registration Date 03-01-2018 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology popup.description2 Analysis of the results of immunocytochemical studies of expression of EMT markers in the cells of the studied lines revealed no significant difference between the expressions of E-cadherin expression. It has also been established that cells of the hormone-sensitive line LNCaP have a significantly higher expression of N-cadherin compared to hormone-refractory DU-145 cells. The cells of both lines of the PC were characterized by high levels of vimentin and pancitotercatin expression, as well as the average level of actin expression. In the cells of LNCaP and DU-145 lines, the expression profile of the miRNAs involved in EMT regulation (miR-200b, -199a, and -21) was also investigated. It was revealed that the characteristic feature of human PC cells insensitive to hormonal therapy are high levels of expression of oncogenic miR-21 and -199. The differences between the levels of miR-200b in the cells of the study lines were not detected. The fact that the cells of the DU-145 line are characterized by a significantly higher proliferative and invasive activity compared to LNCaP cells, as well as a significantly higher level of expression of miR-21 and -199 a, indicates the mesenchymal cell phenotype of this line. Product Description popup.authors Борікун Тетяна Вікторівна Задворний Тарас Володимирович Лихова Олександра Олександрівна Сторчай Дарія Миколаївна popup.nrat_date 2020-04-02 Close