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Information × Registration Number 0218U000060, 0115U001343 , R & D reports Title Molecular-radiation Biotechnology for pharmacological efficacy increasing of medicinal plants in the culture and nature popup.stage_title Head Shylina Yulia Volodymyrivna, к.б.н. Registration Date 16-01-2018 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 Report on SRP: 27 pages, 14 figures, 1 table, 19 references. The object of investigation - medicinal plants Matricaria chamomilla, Silybum marianu, Bidens tripartite, Salvia officinalis. The aim of research - developing of radiation biotechnology increasing produce and isomeric variation of secondary metabolites in medicinal plants in vitro to raise their pharmaceutical value. The methods of investigation - radiobiological, molecular-biological, spectrophotometric, biochemical, physiological, cytological, statistical. It is known that plant radiobiological reactions are followed with genetic and epigenetic changes in satellite DNA. Method of ISSR-amplification between two short inverted repeats was used to investigate molecular effects under radiation exposure. Then amplicones were cut using site-specific HpaII enzyme. DNA was extracted from leaves of vegetated plants originated from radiation exposed seeds. Seeds radiation exposure with doses 1-50 Gy did not lead to genetic polymorphism of ISSR-sequences in microsatellite DNA. Nevertheless it revealed epigenetic changes marked with DNA switching from maintaining to de novo. Qualitative redetection of phenolic components in Matricaria chamomilla extracts was carried out using high-level liquid chromatography. New components in plant extracts originated from radiation exposed seeds did not appear. Only quantitative index of soluble flavonoids was changed. Thus medicinal raw material originated from seeds exposed with both Rh- and ?-radiation is not harmful to human health. Quantitative spectrophotometric analysis revealed 10 Gy dose as an optimal one to achieve maximum flavonoid yield both per 1 g of raw material and area unit. According to obtained data 2 papers were published and 2 conference reports were made. Product Description popup.authors Берестяна Анастасія Миколаївна Гуща Микола Іванович Жук Владислав Вікторович Літвінов Сергій Вячеславович Нестеренко Олена Геннадіївна Пчеловська Світлана Анатоліївна Салівон Анастасія Георгіївна Соколова Дарина Олександрівна Тонкаль Людмила Володимирівна popup.nrat_date 2020-04-02 Close